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KMID : 0881720160310030194
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 3 p.194 ~ p.200
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Simultaneous Determination of Aflatoxins and Ochratoxin A in Pork by LC-MS/MS
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Paek Ock-Jin
Park Song-yi
Park Ki-Hun
Kim Sheen-Hee
Suh Sae-Jung
Yoon Hae-Jung
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Abstract
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Aflatoxins and ochratoxin A (AFTs and OTA) are secondary fungal metabolites produced by several moulds, mainly by Aspergillus flavus by Aspergillus ochraceus and Penicillium verrucosum, and these toxins can be transferred to animals and humans through the ingestion of contaminated feed and food. This study was to develop the analytical method for determination the levels of AFTs (B1, B2, G1 and G2) and OTA in pork. The AFTs and OTA were analyzed simultaneously by electrospray ionization in positive ion mode and mass reaction monitoring (MRM) after solid phase extract (SPE) columns clean-up. Performance characteristics, such as accuracy, precision, linear range, limit of detection (LOD) and quantification (LOQ), were also determined. Matrix-matched standard calibration was used for quantification, obtaining the recoveries in the range of 67.3~108.2% with the relative standard deviations of < 20%. Limits of detection and quantification were also estimated, obtaining the limits of quantification ranged in 0.7~1.3 μg/kg. The results of the inter-day study, which was performed with pork samples for 3 days, showed an accuracy of 92.0~109.9%. The precisions (expressed as relative standard deviation values) for the inter day variation were 2.6~17.8%. The method developed in this study was able to carry out the analysis with the satisfactory intensity and accuracy.
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KEYWORD
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total aflatoxins, ochratoxin A, pork, LC-MS/MS
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